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Titlebook: Germ Cell Protocols; Volume 2: Molecular Heide Schatten Book 2004 Humana Press 2004

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發(fā)表于 2025-3-21 18:35:25 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Germ Cell Protocols
副標(biāo)題Volume 2: Molecular
編輯Heide Schatten
視頻videohttp://file.papertrans.cn/385/384309/384309.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Germ Cell Protocols; Volume 2: Molecular  Heide Schatten Book 2004 Humana Press 2004
描述The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits st- ies of fertility and infertility in mammals including humans. External in vitro fertilization, now a common technique in assisted fertilization has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organi
出版日期Book 2004
版次1
doihttps://doi.org/10.1385/1592597416
isbn_softcover978-1-61737-457-9
isbn_ebook978-1-59259-741-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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Eurodollars and International Banking reporter gene to tag the gene either in a construct (transgenesis) or in the genome (homologous recombination). Transgenes have been generated using different regions of the . gene promoter to drive expression of either lacZ or GFP, which allows the activity of the . promoter to be followed in livi
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https://doi.org/10.1007/978-3-642-69906-1). It is used to study individual genes, gene families, and also for genomewide screenings (.). In a RNAi experiment . is exposed to double-stranded RNA that corresponds to the sequence of a specific mRNA. This triggers a series of enzymatic processes, resulting in a specific degradation of this tar
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https://doi.org/10.1007/978-3-322-89332-1minate between multiple fluorophores. Recently developed techniques, such as confocal (.) or multiphoton imaging (.), permit optical sectioning of intact specimens. These may be collected as stacks of images at different focal depths to obtain three-dimensional (3D) structural data. Stacks of images
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Hans J. Kleinsteuber,Torsten Rossmanniculture, biomedicine, and basic research. Based on the source of donor cells, NT can be classified into embryonic cell NT and somatic cell NT. Somatic cell NT was first reported in 1996 (.) and includes more practical applications. Most importantly, it provides a promising method for producing tran
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