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Titlebook: Exocytosis and Endocytosis; Andrei I. Ivanov Book 2014Latest edition Springer Science+Business Media New York 2014 biochemical assays.cult

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發(fā)表于 2025-3-21 16:58:09 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Exocytosis and Endocytosis
編輯Andrei I. Ivanov
視頻videohttp://file.papertrans.cn/319/318627/318627.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Exocytosis and Endocytosis;  Andrei I. Ivanov Book 2014Latest edition Springer Science+Business Media New York 2014 biochemical assays.cult
描述.Focused on one of the most important and fascinating fields of modern biology, .Exocytosis and Endocytosis, Second Edition. presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis .in vitro. and .in vivo.. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerful .in vivo. imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Highly practical and clearly written, .Exocytosis and Endocytosis, Second Edition. will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and
出版日期Book 2014Latest edition
關(guān)鍵詞biochemical assays; cultured cells; endocytosis; exocytosis; live cell imaging; vesicular trafficking
版次2
doihttps://doi.org/10.1007/978-1-4939-0944-5
isbn_softcover978-1-4939-4694-5
isbn_ebook978-1-4939-0944-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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發(fā)表于 2025-3-22 00:06:51 | 只看該作者
Systematic Analysis of Endocytosis by Cellular Perturbationse plasma membrane serves the internalization of ligands and receptors and leads to their degradation or recycling. A number of distinct mechanisms have been described over the years, several of which are only partially characterized in terms of mechanism and function. These are often referred to as
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Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptidese limited by the ability to identify protein ubiquitination sites. Unbiased high-throughput screening methods are necessary to discover novel ubiquitination sites that play important roles in cellular regulation. Here, we describe an immunopurification approach that enriches ubiquitin remnant-contai
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Use of Kaede and Kikume Green–Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors these important drug targets. In the past, the green fluorescent protein and its derivatives have been widely used as fluorescent tags. More recently, it was reported that photoconvertible fluorescent proteins (PCFPs) such as Kaede or Kikume green–red protein could also be used as fluorescent tags
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發(fā)表于 2025-3-23 06:24:13 | 只看該作者
HaloTag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cellsuli. Defects in any of these processes can lead to organelle dysfunction and have been associated with various inherited and age-related disorders. Progress in this field continues to be driven by advances in live-cell imaging techniques. This chapter provides detailed protocols for the use of HaloT
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