Titlebook: Exocytosis and Endocytosis; Andrei I. Ivanov Book 2014Latest edition Springer Science+Business Media New York 2014 biochemical assays.cult

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SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cellshelial cells, it is critical to develop techniques that permit the selective observation of newly synthesized populations of these proteins. The SNAP tag system permits the detection and visualization of distinct spatially and temporally defined cohorts of tagged proteins. Thus, this technique is es

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Analysis of Protein Dynamics with Tandem Fluorescent Protein Timersently we described a novel class of FTs called tandem fluorescent protein timers (tFTs). Each tFT is a tandem fusion of two different conventional fluorescent proteins having distinct kinetics of fluorophore maturation. tFTs suitable for studying protein dynamics on different scales can be generated

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https://doi.org/10.1007/978-3-476-04927-8ched to the N-terminus and served as a FRET donor. The biarsenical dye FlAsH, served as a FRET acceptor, was bound to a short tetracysteine motif positioned in the linker domain of SNAP-25. CSNAC can report real-time FRET changes when the Syntaxin soluble domain is added in vitro.

Pituitary-Gland

Klaus Niederdrenk,Harry Yserentantning peptides to facilitate downstream mass spectrometry (MS) identification of lysine ubiquitination sites. This approach can be utilized to identify ubiquitination sites from proteins in a complex mixture.

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,Zusammenfassung: dieses Essential in Kürze,in a circular protein. Circularization of bioengineered linear substrate proteins can indeed confer the desirable properties associated with circular proteins. Here, we describe how cells can be manipulated to secrete circularized proteins for substrates of choice via sortase-mediated circularization in the lumen of the endoplasmic reticulum.

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https://doi.org/10.1007/978-3-322-81900-0erty. Here, we describe a detailed protocol for density gradient fractionation of various mammalian subcellular vesicles, including endoplasmic reticulum (ER), Golgi apparatus, endosomes, and lipid rafts, as well as apical and basolateral membranes of polarized epithelial cells.