Titlebook: Ewing Sarcoma; Methods and Protocol Florencia Cidre-Aranaz,Thomas G. P. Grünewald Book 2021 Springer Science+Business Media, LLC, part of S

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Erdogan Madenci,Ibrahim Guven,Bahattin Kiliceased from tumor cells or in response to tumor growth can be used for quantification of the tumor burden. However, only a fraction of pediatric tumors has none of these serum markers, but tumor-specific genetic alterations represent reliable alternatives. Here we describe a method for using genomic

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https://doi.org/10.1007/978-4-431-73464-2However, the first steps of the diagnostic process are crucial to achieve the maximum possible diagnostic performance. In this chapter we will review how to handle the diagnostic specimen from its collection, how to prepare it for diagnosis, how to make a complete pathology report, and provide guida

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https://doi.org/10.1007/978-3-319-56227-8a variety of chimeric genes. Reference laboratories are increasingly implementing NGS-based techniques to overcome several limitations of conventional singleplex determinations. We have been early adopters of a targeted-RNA sequencing method based on Anchored multiplex PCR, which allows assessing se

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Uwe Zerbst,Mauro Madia,Carla Beckmann viral transduction have revolutionized the field of molecular biology and paved the ground for various gene-editing strategies such as TALEN, zinc finger nucleases, and ultimately CRISPR. In Ewing sarcoma (EwS), almost as many genes are repressed by the expression of EWSR1-FLI1 as are upregulated b

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Acoustic Emission and Barkhausen Pulses,f these DNA sequences is especially paramount for its main driver mutation is a fusion transcription factor that binds different motifs than its wild-type constituents. Here, we describe the process of analyzing the enhancer activity of regulatory DNA sequences using transfection-based dual-lucifera

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https://doi.org/10.1007/978-3-031-13498-2ssion/cell division, cell death, and cellular senescence. Deregulation of cell proliferation is a key feature of cancer cells, making assessment of proliferation a central methodological issue in cancer research. Especially in Ewing sarcoma (EwS) that exhibit a high proliferative capacity, experimen

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