Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

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書目名稱Estrogen Receptors影響因子(影響力)




書目名稱Estrogen Receptors影響因子(影響力)學(xué)科排名




書目名稱Estrogen Receptors網(wǎng)絡(luò)公開度




書目名稱Estrogen Receptors網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Estrogen Receptors被引頻次




書目名稱Estrogen Receptors被引頻次學(xué)科排名




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,Immunoblot Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 /GPERlational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immunoblotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estro

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Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Responsls, protein–protein interaction, and protein modifications in cells and tissues. The proximity ligation assay (PLA), a method of detection that combines immunologic and PCR-based approaches, was developed to overcome some of the drawbacks that are inherent with other detection methods. The PLA allow

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Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR, measuring estrogen receptor transcript levels as well as gene expression changes in response to estrogen stimulation as it is quick, accurate, and robust and allows the measurement of gene expression in a variety of tissues and cells. This chapter describes the protocols used for RNA extraction and

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The Use of ERE-Luc Reporter Mice to Monitor Estrogen Receptor Transcriptional Activity in a ,-Temposociated with the menopause still represents a sort of sought after the “Holy Grail.”.Nowadays, the combination of estrogens and selective estrogen receptor modulators (SERMs), a class of compounds with a mixed agonist/antagonistic activity on the estrogen receptor (ER) in various tissues, represent