Titlebook: Estrogen Receptors; Methods and Protocol Kathleen M. Eyster Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under

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An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta,en and activate transcription through direct or indirect interactions with DNA. Revealing their interactions with the chromatin is key to understanding their transcriptional activities and their biological functions. Chromatin-immunoprecipitation followed by sequencing (ChIP-Seq) is a powerful techn

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Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation,n responses to intra- and extracellular signals. Moreover, dysregulated mRNA translation is a common feature in disease states, including neurological disorders and cancer. Yet, most studies of gene expression focus on analysis of mRNA levels, leaving variations in translational efficiencies largely

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Whole-Genome Genotyping Using DNA Microarrays for Population Genetics,–31, 2015). Tools to measure genetic variation have matured significantly throughout this advancement in knowledge (Lenoir and Giannella. J Biomed Discov Collab 1:11, 2006; Marzancola et al. Methods Mol Biol 1368:161–178, 2016). In this chapter, the focus is on the laboratory methods developed to pe

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Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectroms, tissues, biological fluids, secretome, etc.) is a useful strategy to identify proteins and analyze their changes. Samples processed through a gel-free approach provide a simple method for protein separation and profile comparison of different conditions, such as using fewer steps in the protocol,

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Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells,nd can also function as biomarkers. Here, we describe a method for robust characterization of estrogen-regulated microRNA profiles. The activity of estrogen is mediated by two nuclear receptors, estrogen receptor alpha and estrogen receptor beta, and a transmembrane G-protein coupled estrogen recept

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Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and its Co-crystalhis protocol describes SMYD2 molecular cloning and purification and crystallization of SMYD2 in complex with an ERα peptide. Recombinant SMYD2 is constructed and overexpressed in . cells. After release from the cells by French Press, SMYD2 is purified to apparent homogeneity with multiple chromatogr

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Nietzsche, Anti-Semitism and Mass Murder,PB, the conserved motif within the DBD activates the . gene. When this motif was mutated, the activation of . was suppressed significantly. This chapter also describes the use of a phospho-peptide antibody (αP-S216) in the chromatin immunoprecipitation (ChIP) assay, and the co-immunoprecipitation (Co-IP) assay visualized by Western blot analysis.