Titlebook: Epigenetic Reprogramming During Mouse Embryogenesis; Methods and Protocol Katia Ancelin,Maud Borensztein Book 2021 Springer Science+Busines

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Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN,cations in germ cells isolated from mouse embryos from 12.5 up to 18.5?days postfertilization. This approach can be applied to as low as 100 isolated germ cells, allowing the generation of multiple genome-wide profiles from the cells obtained from a single embryo.

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Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-Seq,f as minimal as a single blastomere. Here, we describe a single-cell/single-embryo RNA sequencing (RNA-seq) method, from embryo collection to sample validation steps prior to DNA library preparation and sequencing. Key quality controls and external Spike-In normalization approaches are also detailed.

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遺忘

津貼

,Altern als psychologischer Proze?,ation of KI animals are included. We also describe two independent CRISPR/Cas9 delivery methods to produce gene-edited animals starting from zygote-stage embryos, based either on cytoplasmic injection or electroporation.

Stress