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Titlebook: Epigenetic Reprogramming During Mouse Embryogenesis; Methods and Protocol Katia Ancelin,Maud Borensztein Book 2021 Springer Science+Busines

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發(fā)表于 2025-3-28 16:12:00 | 只看該作者
42#
發(fā)表于 2025-3-28 19:06:20 | 只看該作者
https://doi.org/10.1007/978-3-322-81707-5e method describes the different steps from coverslips and FISH probe preparation to inner cell mass isolation and immunofluorescence followed by RNA-FISH. Furthermore, this is applicable to earlier developmental stages and other mammalian species provided little technical adjustments.
43#
發(fā)表于 2025-3-29 00:28:27 | 只看該作者
https://doi.org/10.1007/978-3-642-60347-1ina-associated domain (LAD) maps. This low-input method can be adapted for other proteins of interest to faithfully profile their genomic interaction, allowing us to interrogate the chromatin dynamics and nuclear organization during the early mammalian development.
44#
發(fā)表于 2025-3-29 04:25:34 | 只看該作者
,Analytische L?sungen mit Beispielen,plantation development. This approach capitalized with the limited amount of material available at these developmental stages. It also provides new research avenues, such as the study of mutant embryos for further functional studies.
45#
發(fā)表于 2025-3-29 08:29:04 | 只看該作者
46#
發(fā)表于 2025-3-29 11:57:49 | 只看該作者
Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining ae method describes the different steps from coverslips and FISH probe preparation to inner cell mass isolation and immunofluorescence followed by RNA-FISH. Furthermore, this is applicable to earlier developmental stages and other mammalian species provided little technical adjustments.
47#
發(fā)表于 2025-3-29 15:53:20 | 只看該作者
48#
發(fā)表于 2025-3-29 19:59:36 | 只看該作者
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發(fā)表于 2025-3-30 02:31:31 | 只看該作者
Otto Blume,Wolfgang Plum,Gerhard Naegelewe describe the different steps of production and storage of the imaging device as well as its use for live imaging of mouse preimplantation embryos expressing fluorescent reporters from genetically modified alleles or after in vitro transcribed mRNA transfer by microinjection or electroporation.
50#
發(fā)表于 2025-3-30 07:23:29 | 只看該作者
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