Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

無能性

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cellsrdinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.

insolence

FATAL

對(duì)手

Perry J. Samson,Jennie L. Moodyipid-based transfection systems. Herein we describe a forward transfection protocol which we routinely use to achieve upwards of 70% transfection efficiency rates in mEpiSCs. Our protocol also includes a suggested transfection timeline and details pertaining to the techniques we use to validate transfection success.

雄偉

動(dòng)機(jī)

W. E. Davis,A. R. Olsen,B. T. Didier process up to 96 cells from a single run with custom library preparation. The method enables detection of Transcription Start Site (TSS) at the single-cell resolution yielding a more comprehensive overview of gene regulatory elements governing in the EpiSC-like cell (EpiLC) including non-polyadenylated RNA and enhancer RNA activities.

白楊

暫時(shí)休息

Volatile Organic Compounds (VOCs) Control,persed colonies, have an increased proliferation rate, and a predisposition to neural fate. EPL cells can be used to study the cell states along the pluripotency continuum, peri-implantation embryogenesis, and as a starting point for efficient neuronal differentiation.

Devastate

Glaci冰

L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Plurpersed colonies, have an increased proliferation rate, and a predisposition to neural fate. EPL cells can be used to study the cell states along the pluripotency continuum, peri-implantation embryogenesis, and as a starting point for efficient neuronal differentiation.