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Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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發(fā)表于 2025-3-21 17:41:38 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Epiblast Stem Cells
副標題Methods and Protocol
編輯Pierre Osteil
視頻videohttp://file.papertrans.cn/314/313140/313140.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license
描述This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful .Method in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Epiblast Stem Cells: Methods and Protocols. serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast..
出版日期Book 2022
關(guān)鍵詞EpiSC; Human developmental steps; Pluripotent stem cells; Epiblast-like cells; Primordial germ cells; Bio
版次1
doihttps://doi.org/10.1007/978-1-0716-2281-0
isbn_softcover978-1-0716-2283-4
isbn_ebook978-1-0716-2281-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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沙發(fā)
發(fā)表于 2025-3-21 23:27:08 | 只看該作者
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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313140.jpg
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發(fā)表于 2025-3-22 05:10:33 | 只看該作者
978-1-0716-2283-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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發(fā)表于 2025-3-22 12:41:44 | 只看該作者
Epiblast Stem Cells978-1-0716-2281-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Air Pollution Effects on Biodiversityotency is a continuum of different states between the two extremes of na?ve embryonic stem cells (ESCs) and primed Epiblast Stem Cells (EpiSCs), which resemble the pre/peri-?and post- implantation embryo, respectively. The transition from na?ve to primed pluripotency can be induced by growing na?ve
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發(fā)表于 2025-3-23 04:20:36 | 只看該作者
https://doi.org/10.1007/978-1-4757-4465-1 development of a mouse embryo allows us to identify the genetic basis of errors of development in animal disease models. Immunofluorescence is a powerful technique to study the localization and variation in expression pattern of specific proteins in cells, tissues, and organs. Detecting the antigen
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發(fā)表于 2025-3-23 06:03:40 | 只看該作者
Perry J. Samson,Jennie L. Moodyue to innate differences in cellular characteristics, the efficiency of lipid-based transfection is variable across cell types. Pluripotent cells which exist in a “primed” state such as human embryonic stem cells (hESCs) and mouse epiblast stem cells (mEpiSCs) are notorious for being refractory to l
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