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Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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樓主: Iridescent
11#
發(fā)表于 2025-3-23 12:03:19 | 只看該作者
12#
發(fā)表于 2025-3-23 17:09:26 | 只看該作者
PPI-Theory for Particle Dispersion microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficie
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發(fā)表于 2025-3-23 19:04:04 | 只看該作者
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發(fā)表于 2025-3-23 23:39:33 | 只看該作者
Air Quality Modeling in Complex Terrainsify putative regulatory regions, determine dynamics and mechanisms of transcription factors when coupled with ChIP-seq and predict interactions between proteins and chromatin. Compared to previous methods, MNase-seq and DNase-seq, ATAC-seq requires only 50,000 cells, orders of magnitude fewer cells.
15#
發(fā)表于 2025-3-24 04:29:30 | 只看該作者
W. E. Davis,A. R. Olsen,B. T. Didierlk populations. However most of the methods focus on the 3′-end of polyadenylated transcripts using droplet-based technology. To achieve complete transcriptome, we describe single-cell 5′-end transcriptome protocol with random primed-cDNA harvesting on the Fluidigm C1? platform which can isolate and
16#
發(fā)表于 2025-3-24 08:17:04 | 只看該作者
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發(fā)表于 2025-3-24 13:28:20 | 只看該作者
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發(fā)表于 2025-3-24 18:18:35 | 只看該作者
P. Grossi,G. Graziani,C. Ceruttills (ESCs) aggregated in suspension culture are able to form 3D embryo-like structures called gastruloids that mimic features of the gastrulation process. Recent findings indicate that gastruloid formation efficiency decreases as pluripotency progresses from na?ve to primed state, and suggest that g
19#
發(fā)表于 2025-3-24 20:23:13 | 只看該作者
G. Kallos,V. Kotroni,K. Lagouvardosmbryos. The basis of this protocol mimicking the in vivo gastrulation process makes a contrast with those using sequential administration of pharmacological molecules and recombinant signaling proteins even at nonphysiological levels. In the experimental setup, EpiSCs are first freed from the dish-a
20#
發(fā)表于 2025-3-24 23:19:01 | 只看該作者
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