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Titlebook: Enzymes in Nonaqueous Solvents; Methods and Protocol Evgeny N. Vulfson,Peter J. Halling,Herbert L. Holl Book 2001 Humana Press 2001 biochem

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41#
發(fā)表于 2025-3-28 15:48:23 | 只看該作者
42#
發(fā)表于 2025-3-28 18:50:19 | 只看該作者
Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopyis reasonably assumed that changes in activity and specificity of enzymes are mainly ascribed to changes in the higher structures of enzymes. Therefore, analysis of enzyme structure is important for the selection of the solvent, but methods to analyze dispersed or immobilized enzymes in organic solvents are rather limited.
43#
發(fā)表于 2025-3-29 02:15:48 | 只看該作者
44#
發(fā)表于 2025-3-29 07:04:39 | 只看該作者
Evolution of Project Managementctrostatic and hydrogen-bonding interactions among the surface residues of the protein in organic solvents (.). Despite this rigidity, enzymes remain active and selective in organic solvents, and this has led to a large number of applications that have impacted the chemical, pharmaceutical, and poly
45#
發(fā)表于 2025-3-29 09:35:35 | 只看該作者
Agile Development with ICONIX Processof photo-crosslinkable resin prepolymers having photosensitive functional groups, irradiation of near-ultraviolet (UV) light (maximum intensity at 365 nm) induces radical polymerization of the prepolymers and gel formation completes within a few minutes. Various types of prepolymers with different c
46#
發(fā)表于 2025-3-29 12:26:14 | 只看該作者
47#
發(fā)表于 2025-3-29 19:35:32 | 只看該作者
From Ad Hoc to Agility: A General Framework,orbed onto insoluble materials, copolymerized with a reactive monomer, encapsulated in gels, crosslinked with a bifunctional reagent, covalently bound to an insoluble carrier (.) or entrapped within an insoluble gel matrix of natural or synthetic resin (.).
48#
發(fā)表于 2025-3-29 23:11:24 | 只看該作者
https://doi.org/10.1007/978-3-658-41332-3r the purpose of applying proteins to biomedical and biotechnological processes (.-.). In the biomedical field, the purpose of the chemical modification of enzymes with PEG includes the reduction of their immunoreactivity and immunogenicity as well as the prolongation of the plasma half-life of the
49#
發(fā)表于 2025-3-30 00:04:20 | 只看該作者
50#
發(fā)表于 2025-3-30 04:34:49 | 只看該作者
https://doi.org/10.1007/978-981-10-3358-2ic synthesis and numerous examples also in the fields of food-related conversions and analysis. However, enzymes show a lower catalytic efficiency (up to three or four orders of magnitude) when employed in organic solvents rather than in aqueous buffer. It is evident that such behavior represents an
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