Titlebook: Engineering and Manufacturing for Biotechnology; Marcel Hofman,Philippe Thonart Book 2002 Springer Science+Business Media Dordrecht 2002 D

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Prosaic

Enzymic Solubilisation of Proteins from Tropical Tuna Using Alcalase and Some Biological Properties ular weight distribution of peptides using size exclusion chromatography (SEC). Preliminary results showed that tuna protein hydrolysates performed effectively as cellular growth factors on fibroblastic cells and as nitrogen sources in microbial growth media. The presence of “secretagogue” molecules

廢止

藐視

Macroscopic Modelling of Bioprocesses with a View to Engineering Applicationsramework of system of mass balances based on macroscopic reaction schemes is recalled and a new general kinetic model structure is presented and analysed. A general methodology for the parameter identification (kinetic and pseudo-stoichiometric coefficients) is summarised. Necessary conditions of va

DEAF

A Model Discrimination Approach for Data Analysis and Experimental Designal design. Results of closed-loop controlled steady-state fermentations with the methylotrophic yeast . are used to clearly discriminate the “right” model out of a group of 10 competing models (53% model probability). Using the identified model the kinetics of batch and fed-batch fermentations with

DEAF

Model Based Sequential Experimental Design for Bioprocess Optimisation — an Overviewates necessary to obtain high product yields. Knowledge and data based hybrid modelling techniques are suitable to build such models. Two concepts of model based experimental design called direct and indirect experimental design are established. The direct design focuses on experiments being optimal

顧客

Metabolic Flux Modelling as a Tool to Analyse the Behavior of a Genetically Modified Strain of thermodynamic analysis of central metabolism under anaerobic conditions. Starting from a redundant set of measured rates obtained from batch cultures on glucose or fructose as carbon source, an original data reconciliation technique associated with the calculation of metabolic flux is used. Comparat

entreat

Metabolic Investigation of an Anaerobic Cellulolytic Bacterium: abolic pathways that have been proved to exist and anabolic pathways. The measured conversion yields are correlated in terms of metabolic flux distribution using a mathematical technique of yields calculation associatedwith a methodology of data reconciliation. This approach validates the metabolic

POLYP

Crossflow Ultrafiltration of , Fermentation Medium to Separate Protease Enzymesnvestigated in a crossflow ultrafiltration system by using 30 000 Da MWCO polysulphone membrane. The effects of initial enzyme concentration and recirculation velocity on the permeate flux, on the total resistance, and on the recovery yield of SAP in the permeate were investigated. High permeate flu