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Titlebook: Emerging Model Organisms; Wei Wang,Nicolas Rohner,Yongfu Wang Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive l

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發(fā)表于 2025-3-21 18:29:42 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Emerging Model Organisms
編輯Wei Wang,Nicolas Rohner,Yongfu Wang
視頻videohttp://file.papertrans.cn/309/308313/308313.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Neuromethods
圖書封面Titlebook: Emerging Model Organisms;  Wei Wang,Nicolas Rohner,Yongfu Wang Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive l
描述.This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, ?setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging,? Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the?.Neuromethods.?series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.?..Authoritative and cutting-edge,?.Emerging Model Organisms .aims to be a?useful practical guide to researches to help further their study in this field.?.
出版日期Book 2023
關(guān)鍵詞Tol2-mediated transgenesis; CRISPR; Nothobranchius furzeri; regenerative medicine; gene knockdown
版次1
doihttps://doi.org/10.1007/978-1-0716-2875-1
isbn_softcover978-1-0716-2877-5
isbn_ebook978-1-0716-2875-1Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Whole-Brain Clearing and Immunolabelling in the African Killifish biological processes. Yet, the opaqueness of fixed samples prevents the visualization of large volumes of tissue. Tissue clearing allows to match the refractive indices of biological specimens with the mounting media, allowing light penetration and imaging of millimetric depths within tissues. We p
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The Chromatin Immunoprecipitation (ChIP) Protocol for African Turquoise Killifishrofiling their genomic distribution on a genome-wide scale is of crucial significance for decoding the underlying gene regulatory networks. Chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) is a powerful and extensively employed approach for studying in vivo protein
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EdU Labeling of Proliferating Cells in the African Killifish context of development, wound healing, and regeneration. In this chapter, we present a protocol to perform intraperitoneal injection of EdU to label DNA of dividing killifish cells. After a short in vivo labeling pulse of EdU, we list detailed instructions and important considerations for tissue co
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