Titlebook: Emerging Model Organisms; Wei Wang,Nicolas Rohner,Yongfu Wang Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive l

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書目名稱Emerging Model Organisms影響因子(影響力)




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Whole-Brain Clearing and Immunolabelling in the African Killifish biological processes. Yet, the opaqueness of fixed samples prevents the visualization of large volumes of tissue. Tissue clearing allows to match the refractive indices of biological specimens with the mounting media, allowing light penetration and imaging of millimetric depths within tissues. We p

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The Chromatin Immunoprecipitation (ChIP) Protocol for African Turquoise Killifishrofiling their genomic distribution on a genome-wide scale is of crucial significance for decoding the underlying gene regulatory networks. Chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) is a powerful and extensively employed approach for studying in vivo protein

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EdU Labeling of Proliferating Cells in the African Killifish context of development, wound healing, and regeneration. In this chapter, we present a protocol to perform intraperitoneal injection of EdU to label DNA of dividing killifish cells. After a short in vivo labeling pulse of EdU, we list detailed instructions and important considerations for tissue co

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