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Titlebook: Drosophila Oogenesis; Methods and Protocol Diana P. Bratu,Gerard P. McNeil Book 2015 Springer Science+Business Media New York 2015 RNA-prot

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發(fā)表于 2025-3-21 19:59:57 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Drosophila Oogenesis
副標(biāo)題Methods and Protocol
編輯Diana P. Bratu,Gerard P. McNeil
視頻videohttp://file.papertrans.cn/283/282977/282977.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Drosophila Oogenesis; Methods and Protocol Diana P. Bratu,Gerard P. McNeil Book 2015 Springer Science+Business Media New York 2015 RNA-prot
描述.This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, Drosophila. Oogenesis: Methods and Protocols. aims to ensure successful results in the further study of this vital field..
出版日期Book 2015
關(guān)鍵詞RNA-protein interactions; cell death; fly ovary; generating mutant clones; genome wide gene expression a
版次1
doihttps://doi.org/10.1007/978-1-4939-2851-4
isbn_softcover978-1-4939-4933-5
isbn_ebook978-1-4939-2851-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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1064-3745 ation advice from the experts.This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analys
板凳
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https://doi.org/10.1007/978-3-642-97843-2H method to label small RNAs (200–500 nucleotides in length) that are enriched in nuclear bodies in . ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other . tissues, and to abundant mRNAs such as histone transcripts.
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https://doi.org/10.1007/978-3-642-98102-9al new mRNA target(s) of the . master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA–protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.
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https://doi.org/10.1007/978-3-642-98102-9e. The newly transcribed nascent RNA is then isolated and cloned using a small RNA cloning protocol. Although it is time-consuming, the global run-on method allows the user to profile the position, orientation and amount of transcriptionally engaged RNA polymerases across the genome, therefore providing a snapshot of genome-wide transcription.
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Global Run-On Sequencing (GRO-seq) Library Preparation from , Ovaries,e. The newly transcribed nascent RNA is then isolated and cloned using a small RNA cloning protocol. Although it is time-consuming, the global run-on method allows the user to profile the position, orientation and amount of transcriptionally engaged RNA polymerases across the genome, therefore providing a snapshot of genome-wide transcription.
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