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Titlebook: Drosophila Oogenesis; Methods and Protocol Diana P. Bratu,Gerard P. McNeil Book 2015 Springer Science+Business Media New York 2015 RNA-prot

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發(fā)表于 2025-3-23 10:38:13 | 只看該作者
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,Bioinformatics Analysis to Identify RNA–Protein Interactions in Oogenesis,al new mRNA target(s) of the . master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA–protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.
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Culturing , Egg Chambers and Investigating Developmental Processes Through Live Imaging,ent microscopy. This technique is suitable for observation of early- or late-stage egg development, and can be adapted to study a variety of cellular, molecular, or developmental processes. Ongoing analysis of oogenesis in living egg chambers has tremendous potential for discovery of new developmental mechanisms.
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https://doi.org/10.1007/978-3-642-97360-4. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.
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