Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring

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Elizabeth M.J. Gillam,Janine N. Copp,David AckerleIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolutiond the need to create an effective library from those variants. Generation of a maximally diverse gene library is particularly important when employing nontargeted mutagenesis strategies such as error-prone PCR (epPCR), which seek to explore very large areas of sequence space. Here we present compreh

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Generation of Effective Libraries by Neutral Drift accessible sequence space by repeated rounds of mutagenesis and selection to maintain wild-type function. Mutations that are largely neutral for the native function accumulate, and those that are highly detrimental are purged, yielding a library of high diversity and quality. This technique is usef

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Site-Saturation Mutagenesis by Overlap Extension PCRorporation of degenerate base combinations at specific codon locations, giving rise to a precise series of amino acid substitutions in the encoded protein. Here we describe a simple and efficient overlap PCR protocol for the introduction of degenerate bases at either single or multiple codon locatio

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