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Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring

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書目名稱Directed Evolution Library Creation
副標題Methods and Protocol
編輯Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle
視頻videohttp://file.papertrans.cn/281/280663/280663.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring
描述.Directed Evolution Library Creation: Methods and Protocols, Second Edition .presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists o
出版日期Book 2014Latest edition
關鍵詞deletion mutations; directed evolution; gene variant libraries; insertion mutations; mutagenesis; genetic
版次2
doihttps://doi.org/10.1007/978-1-4939-1053-3
isbn_softcover978-1-4939-5329-5
isbn_ebook978-1-4939-1053-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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Robert D. Poodiack,William E. Woodth at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I diges
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Robert D. Poodiack,William E. Woodf the DNA library nor the number of DNA changes has any limits. Furthermore, with the costs of synthetic DNA dropping every year, after an initial investment is made in the oligonucleotides, these can be exchanged for alternative ones with different sequences at any point in the process, fully explo
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1064-3745 design and analysis of mutant libraries. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists o978-1-4939-5329-5978-1-4939-1053-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Random Mutagenesis by Error-Prone Pol Plasmid Replication in ,nesis method that is based on error-prone replication of a ColE1 plasmid bearing the gene of interest. Compared to other in vivo mutagenesis methods, this plasmid-targeted approach allows increased mutation loads and facilitates iterative selection approaches. We also describe the mutation spectrum
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Restriction Enzyme-Mediated DNA Family Shufflingth at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I diges
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