找回密碼
 To register

QQ登錄

只需一步，快速開始

掃一掃，訪問微社區(qū)

打印 上一主題 下一主題

Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring

[復制鏈接]
查看: 41314|回復: 61
樓主
發(fā)表于 2025-3-21 19:22:30 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Directed Evolution Library Creation
副標題Methods and Protocol
編輯Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle
視頻videohttp://file.papertrans.cn/281/280663/280663.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring
描述.Directed Evolution Library Creation: Methods and Protocols, Second Edition .presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists o
出版日期Book 2014Latest edition
關鍵詞deletion mutations; directed evolution; gene variant libraries; insertion mutations; mutagenesis; genetic
版次2
doihttps://doi.org/10.1007/978-1-4939-1053-3
isbn_softcover978-1-4939-5329-5
isbn_ebook978-1-4939-1053-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

書目名稱Directed Evolution Library Creation影響因子(影響力)




書目名稱Directed Evolution Library Creation影響因子(影響力)學科排名




書目名稱Directed Evolution Library Creation網(wǎng)絡公開度




書目名稱Directed Evolution Library Creation網(wǎng)絡公開度學科排名




書目名稱Directed Evolution Library Creation被引頻次




書目名稱Directed Evolution Library Creation被引頻次學科排名




書目名稱Directed Evolution Library Creation年度引用




書目名稱Directed Evolution Library Creation年度引用學科排名




書目名稱Directed Evolution Library Creation讀者反饋




書目名稱Directed Evolution Library Creation讀者反饋學科排名




單選投票, 共有 0 人參與投票
 

0票 0%

Perfect with Aesthetics
 

0票 0%

Better Implies Difficulty
 

0票 0%

Good and Satisfactory
 

0票 0%

Adverse Performance
 

0票 0%

Disdainful Garbage
您所在的用戶組沒有投票權限
沙發(fā)
發(fā)表于 2025-3-21 23:07:31 | 只看該作者
板凳
發(fā)表于 2025-3-22 00:53:30 | 只看該作者
Robert D. Poodiack,William E. Woodth at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I diges
地板
發(fā)表于 2025-3-22 05:58:57 | 只看該作者
Robert D. Poodiack,William E. Woodf the DNA library nor the number of DNA changes has any limits. Furthermore, with the costs of synthetic DNA dropping every year, after an initial investment is made in the oligonucleotides, these can be exchanged for alternative ones with different sequences at any point in the process, fully explo
5#
發(fā)表于 2025-3-22 12:02:51 | 只看該作者
6#
發(fā)表于 2025-3-22 13:30:40 | 只看該作者
7#
發(fā)表于 2025-3-22 20:42:23 | 只看該作者
1064-3745 design and analysis of mutant libraries. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists o978-1-4939-5329-5978-1-4939-1053-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
8#
發(fā)表于 2025-3-22 23:48:10 | 只看該作者
Random Mutagenesis by Error-Prone Pol Plasmid Replication in ,nesis method that is based on error-prone replication of a ColE1 plasmid bearing the gene of interest. Compared to other in vivo mutagenesis methods, this plasmid-targeted approach allows increased mutation loads and facilitates iterative selection approaches. We also describe the mutation spectrum
9#
發(fā)表于 2025-3-23 05:20:12 | 只看該作者
10#
發(fā)表于 2025-3-23 09:20:21 | 只看該作者
Restriction Enzyme-Mediated DNA Family Shufflingth at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I diges
 關于派博傳思  派博傳思旗下網(wǎng)站  友情鏈接
派博傳思介紹 公司地理位置 論文服務流程 影響因子官網(wǎng) 吾愛論文網(wǎng) 大講堂 北京大學 Oxford Uni. Harvard Uni.
發(fā)展歷史沿革 期刊點評 投稿經(jīng)驗總結 SCIENCEGARD IMPACTFACTOR 派博系數(shù) 清華大學 Yale Uni. Stanford Uni.
QQ|Archiver|手機版|小黑屋| 派博傳思國際 ( 京公網(wǎng)安備110108008328) GMT+8, 2025-10-5 19:29
Copyright © 2001-2015 派博傳思   京公網(wǎng)安備110108008328 版權所有 All rights reserved
快速回復 返回頂部 返回列表
延吉市| 华蓥市| 河西区| 苍南县| 新泰市| 额敏县| 津南区| 兴化市| 汕尾市| 福安市| 咸阳市| 泗洪县| 宜城市| 丽水市| 定州市| 秭归县| 西乌珠穆沁旗| 六盘水市| 长海县| 吉木萨尔县| 延庆县| 仙居县| 明溪县| 宁明县| 彩票| 嵊州市| 千阳县| 于田县| 永丰县| 鹿泉市| 北安市| 合江县| 白朗县| 遂溪县| 乡城县| 西吉县| 太保市| 武邑县| 盖州市| 永康市| 正镶白旗|