Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring

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Guglielmo Chiodi,Leonardo Dittafull randomization by making use of carefully designed mutagenic cassettes and an optimized one-pot megaprimer PCR. The method is especially suited to construct libraries of up to ten randomized codons for focused directed evolution, exhibits up to 97 % efficiency in the amplification of mutated ove

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https://doi.org/10.1007/978-1-4939-1053-3deletion mutations; directed evolution; gene variant libraries; insertion mutations; mutagenesis; genetic

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