Titlebook: DNAzymes; Methods and Protocol Gerhard Steger,Hannah Rosenbach,Ingrid Span Book 2022 The Editor(s) (if applicable) and The Author(s), under

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Selection of Aptamer for ,-Methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzymeing to the metalation of MPIX. The DNA aptamers for porphyrin molecules usually display different catalytic functions (termed deoxyribozymes or DNAzymes), which can accelerate such chemical reactions. Inspired by this, an affinity chromatography selection approach was designed for identifying a porp

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PCR Methods for the Generation of Catalytic ssDNAyribozymes, but is particularly important for performing in vitro selections. While the production of single-stranded RNA is straightforward by means of in vitro transcription, the enzymatic production of single-stranded DNA?(ssDNA) on a preparative scale is often difficult. Nevertheless, several me

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Crystallization and Structural Determination of 8–17 DNAzymed significant attention. However, due to their intrinsic difficulties in crystallization and structural determination, only very limited structural information of DNAzymes is available to date. Using co-crystallization with the . Polymerase X (.PolX) protein, we have recently solved a complex struct

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Single-Molecule Kinetic Studies of Nucleic Acids by F?rster Resonance Energy Transfer structures and the challenges specific to single-molecule observation techniques, the data recorded in such experiments must be processed via multiple statistical treatments to finally yield a reliable mechanistic view of the NA dynamics. In this chapter, we propose a comprehensive protocol to anal