Titlebook: DNAzymes; Methods and Protocol Gerhard Steger,Hannah Rosenbach,Ingrid Span Book 2022 The Editor(s) (if applicable) and The Author(s), under

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Peng Zhang,Xiaoyu Ge,Jochen Renzased on their ability to form and change their three-dimensional structure. Binding of divalent ions to specific binding pockets was found to be important for the global structure and function. Here, we present a protocol that allows counting the number of Mn.-binding sites and to determine their di

Fracture

Peng Zhang,Xiaoyu Ge,Jochen Renz obtain well-diffracting single crystals and to solve the phase problem. The absence of structural information impedes the elucidation of the molecular details of biological processes. A particularly intriguing example is the RNA-cleavage catalyzed by the 10–23 deoxyribozyme (DNAzyme). This DNAzyme

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Peng Zhang,Xiaoyu Ge,Jochen Renzlations. However, as DNA or RNA are highly charged molecules, performing such simulations is challenging as to the representation of intramolecular electrostatic interactions and those to solvent molecules and ions. This is particularly true for DNAzymes, where DNA and RNA backbones can come as clos

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https://doi.org/10.1007/978-3-031-00119-2s, for example, oligonucleotides. In order to be applicable, the oligonucleotide has to be labeled site-specifically with paramagnetic tags, the so-called spin labels. Here, we provide a protocol for spin labeling of long oligonucleotides with nitroxides. In the first step, a short and commercially

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Conspiracy