Titlebook: DNA-Protein Interactions; Principles and Proto Beno?t P. Leblanc,Sébastien Rodrigue Book 2015Latest edition Springer Science+Business Media

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Lecture Notes in Computer Scienceusing bulky nucleases such as DNase I. The DNase I footprinting method was developed to take advantage of this fact in the study of DNA-protein interactions: it consists in comparing the pattern of fragments generated by the partial digestion of a DNA sequence in the absence of a protein to that pro

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Mar Marcos,Jose M. Juarez,Gregor Stiglicled DNA with a gel blot, washing, and visualizing through autoradiography. A blot resulting from 1-dimensional SDS-PAGE reveals the molecular weight of the binding proteins. To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electropho

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Olga Dolinina,Igor Bessmertny,Vadim Zhmud and repair. Identifying and examining the nature of these interactions is therefore a crucial prerequisite to understand the molecular basis of how these fundamental processes take place. The application of fluorescence techniques and in particular fluorescence resonance energy transfer (FRET) to p

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https://doi.org/10.1007/978-3-031-06242-1y only a weak sequence preference, making the analysis of their DNA-binding characteristics difficult if not impossible in a standard electrophoretic mobility shift assay (EMSA). In contrast, such proteins often bind prebent DNAs with high affinity and specificity. A synthetic cruciform DNA structur