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Titlebook: DNA Topoisomerases; Methods and Protocol Duncan J. Clarke Book 2009 Humana Press 2009 Cellular functions.Chromosom.Chromosome structure.DNA

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發(fā)表于 2025-3-23 13:00:01 | 只看該作者
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Artificial Intelligence XXXVIIIrticularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.
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發(fā)表于 2025-3-24 00:27:29 | 只看該作者
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發(fā)表于 2025-3-24 06:13:28 | 只看該作者
Yu An,Qing Ye,Jiulin Guo,Ruihai Dongolution, chromosome condensation, and sister chromatid separation. Deficiencies in these enzymes are associated with diseases that result from genome instability. The last 10–15 years has seen a great deal of exciting research in the field of topoisomerase. Here we discuss a selection of the new the
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發(fā)表于 2025-3-24 06:40:55 | 只看該作者
Lecture Notes in Computer Scienceas been used for more than 30 years, the physical mechanism underlying the resolution of topological states remains poorly understood. However, electrophoretic methods remain the most robust and precise techniques for determining the local unwinding of DNA induced by the binding of proteins and smal
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Lecture Notes in Computer Scienceinactive mutant form of the enzyme. In the gel shift assay, the preference for binding to supercoiled plasmid DNA is detected in the presence of linear and nicked forms of the same DNA by a reduction in the mobility of the supercoiled plasmid during electrophoresis in agarose. The more quantitative
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發(fā)表于 2025-3-25 00:36:31 | 只看該作者
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