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Titlebook: DNA Topoisomerases; Methods and Protocol Duncan J. Clarke Book 2009 Humana Press 2009 Cellular functions.Chromosom.Chromosome structure.DNA

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書目名稱DNA Topoisomerases
副標題Methods and Protocol
編輯Duncan J. Clarke
視頻videohttp://file.papertrans.cn/261/260202/260202.mp4
概述Provides an easily accessible reference volume for topoisomerase protocols written by leading researchers in the field.Covers a wide range of assays from cell-based methods and molecular analysis of t
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: DNA Topoisomerases; Methods and Protocol Duncan J. Clarke Book 2009 Humana Press 2009 Cellular functions.Chromosom.Chromosome structure.DNA
描述.Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In .DNA Topoisomerases: Methods and Protocols., experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful .Methods in Molecular Biology?. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls...Cutting
出版日期Book 2009
關(guān)鍵詞Cellular functions; Chromosom; Chromosome structure; DNA; DNA replication; DNA topologies; Eukaryote genom
版次1
doihttps://doi.org/10.1007/978-1-60761-340-4
isbn_softcover978-1-61779-644-9
isbn_ebook978-1-60761-340-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Artificial Intelligence XXXVIIIthod for creating the DNA substrate and using it as a tool to monitor the conformational changes at the topo II DNA gate. We detail how to collect and process fluorescence spectra to determine the FRET efficiency of the DNA substrate.
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Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II,tranded DNA cleavage by monitoring the conversion of covalently closed supercoiled DNA to linear molecules. The assay is simple, straightforward, and does not require the use of radiolabeled substrates.
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Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Hum
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