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Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D

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發(fā)表于 2025-3-25 03:55:00 | 只看該作者
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260188.jpg
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https://doi.org/10.1007/978-3-642-73739-8ation. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.
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發(fā)表于 2025-3-25 14:53:16 | 只看該作者
A Chinese Model of Education in New Zealandlenging. Choosing the appropriate method depends upon the parameters of the experiment, the nature of the molecules to be observed, and the hypothesis to be tested. In this chapter, we review three broad types of visualization: whole-cell fluorescence or immunofluorescence, which is useful for quest
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發(fā)表于 2025-3-25 16:55:55 | 只看該作者
https://doi.org/10.1007/978-981-10-0330-1e course of S-phase, semiconservative DNA synthesis is sequentially initiated and performed at thousands of discrete patches of the DNA helix termed replicons. At any given moment of S-phase, multiple replicons are active in parallel in different parts of the genome. In the last decades, tools and m
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