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Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D

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發(fā)表于 2025-3-21 16:06:42 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱DNA Replication
副標(biāo)題Methods and Protocol
編輯Sonya Vengrova,Jacob Dalgaard
視頻videohttp://file.papertrans.cn/261/260188/260188.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D
描述.Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of. DNA Replication: Methods and Protocols .describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments.?As a volume in the highly successful .Methods in Molecular Biology. series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .DNA Replication: Methods and Protocols, Second Edition .provides a collections of methods intended for newcomers to this research field and for established laboratories..
出版日期Book 2015Latest edition
關(guān)鍵詞DNA replication; archaeal; bacterial; genomes; mammalian; single molecules; yeast
版次2
doihttps://doi.org/10.1007/978-1-4939-2596-4
isbn_softcover978-1-4939-5591-6
isbn_ebook978-1-4939-2596-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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發(fā)表于 2025-3-21 22:35:10 | 只看該作者
Microscopy Techniques to Examine DNA Replication in Fission Yeast,lenging. Choosing the appropriate method depends upon the parameters of the experiment, the nature of the molecules to be observed, and the hypothesis to be tested. In this chapter, we review three broad types of visualization: whole-cell fluorescence or immunofluorescence, which is useful for quest
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發(fā)表于 2025-3-22 03:38:34 | 只看該作者
High-Resolution Analysis of Mammalian DNA Replication Units,e course of S-phase, semiconservative DNA synthesis is sequentially initiated and performed at thousands of discrete patches of the DNA helix termed replicons. At any given moment of S-phase, multiple replicons are active in parallel in different parts of the genome. In the last decades, tools and m
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發(fā)表于 2025-3-22 07:46:37 | 只看該作者
Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing,sites distributed along mammalian chromosomes, yet a given cell uses only a subset of these origins due to inefficient origin activation and regulation by developmental or environmental cues. An impractical consequence of cell-to-cell variations in origin firing is that population-based techniques d
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Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Excnd accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analys
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Measuring Ribonucleotide Incorporation into DNA In Vitro and In Vivo,nsequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.
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