Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan

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Aortic Response to Various Effects,ells of higher eukaryotes to repair this lesion, nonhomologous end-joining (NHEJ) is the most dominant. The biochemical characterization of NHEJ has significantly benefited from in vitro plasmid end-joining assays that can complement and extend information obtained from genetic studies. There is evi

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https://doi.org/10.1007/978-1-4684-3243-5seases. Gene targeting approaches are also useful for studying the mechanisms of homologous recombination. We have developed gene targeting methods that we have specifically used to investigate the mechanisms of recombination in cultured mammalian cells. In this chapter, we describe the generation o

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William D. Wagner,Thomas B. Clarksonth high reproducibility. Specific nicking and loss of a restricted DNA fragment at the site of induced damage is visualized by Southern blot and quantified against a control; since the blot is gene specific, only the damage of interest is measured. Here we show how the assay may be adapted to assess

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Jack C. Geer,William S. Webster the genome is modulated by the DNA sequence, by DNA methylation patterns, by the transcriptional status of the locus, and by chromatin proteins associated with the DNA. The only method currently available to allow a precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated

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Wolfgang Schaper,Dimitri Scholzeous genetic damage has developed. This, in turn, has increased interest in the cellular mechanisms responsible for tumorigenesis, and the need to develop experimental methodologies to investigate these mechanisms. DNA represents a most important cellular target for ultraviolet radiation (UVR), lead

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Eric Allaire,Peter Libby,Alexander W. Clowesver other analytical procedures currently used to measure DNA damage including adaptability, sensitivity and selectivity. This combination of attributes allows for the development of powerful analytical techniques to visualize and quantify specific types of DNA damage in cells and organisms exposed