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Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan

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發(fā)表于 2025-3-21 17:21:59 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱DNA Repair Protocols
編輯Daryl S. Henderson
視頻videohttp://file.papertrans.cn/261/260179/260179.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: DNA Repair Protocols;  Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan
描述The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in
出版日期Book 2006
關(guān)鍵詞DNA; DNA replication; Ligation; PCR; Polyacrylamid-Gelelektrophorese; Quantitative PCR; cell lines; genes; r
版次1
doihttps://doi.org/10.1385/1592599737
isbn_softcover978-1-61737-611-5
isbn_ebook978-1-59259-973-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2006
The information of publication is updating

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Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes,ng ultraviolet (UV) light-induced DNA lesions, may result in the rare, recessively inherited autosomal syndromes xeroderma pigmentosum (XP), Cockayne syndrome (CS), or trichothiodystrophy (TTD). Classical approaches such as somatic cell fusions or microinjection assays have formalized the genetic co
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Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation,ced genomic instability. Perhaps the best characterized is the dynamic production of chromosomal rearrangements in some clonally expanded cells surviving irradiation. In this chapter we provide the protocols for irradiation, cell culture, chromosome analysis, and characterization of the status of ge
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Cytometric Assessment of Histone H2AX Phosphorylation,e. Immunocytochemical detection of phosphorylated H2AX (denoted as γH2AX) thus provides a marker of DSBs. The method presented in this chapter describes the detection of γH2AX for revealing the presence of DSBs, combined with differential staining of cellular DNA for revealing the cell cycle phase.
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Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Celde of cell death. This chapter describes methods to label . DNA strand breaks with fluorochromes for detection by flow or laser scanning cytometry. By staining DNA with a fluorochrome of another color, cellular DNA content is measured concurrently and the bivariate analysis of such a data reveals DN
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In Vitro Rejoining of Double-Strand Breaks in Genomic DNA,nd have helped to characterize several components including DNA-PKcs, Ku, DNA ligase IV, and XRCC4. There is evidence, however, that additional factors involved in NHEJ remain to be characterized. The biochemical characterization of NHEJ in higher eukaryotes has benefited significantly from in vitro
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Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR andask any site-dependent variations in breakage and repair rate or fidelity. We present here a system for analyzing the fate of documented DNA breaks, using the . gene as an example, through application of ligation-mediated PCR. Here, a simple asymmetric double-stranded DNA adapter molecule is ligated
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