Titlebook: Computational Design of Ligand Binding Proteins; Barry L. Stoddard Book 2016 Springer Science+Business Media New York 2016 ligand-binding

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Zusammenfassung der Ergebnisse, protein design with the high-throughput screening of mutagenic libraries of computationally designed proteins is emerging as a general approach for creating binding proteins with programmable binding modes, affinities, and selectivities. The computational step enables the creation of a binding site

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Zusammenfassung der Ergebnisse,luding spatial and temporal control and high selectivity. Here, we describe a general protocol for engineering a protein to be controllable with light via reaction with an exogenously introduced photoisomerizable small molecule and illustrate our protocol with two examples from the literature: the e

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Zusammenfassung der Ergebnisse,a large number of peptide ligands for a protein target. In this chapter, we describe a procedure called “SORTCERY” that can rank the affinities of library members for a target with high accuracy. SORTCERY follows a three-step protocol. First, fluorescence-activated cell sorting (FACS) is used to sor