Titlebook: Chromatin Accessibility; Methods and Protocol Georgi K. Marinov,William J. Greenleaf Book 2023 The Editor(s) (if applicable) and The Author

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Innovations in Monitoring Ocean History two orthogonal approaches to correct for this bias, the “corrected cut–uncut” and the “cut–all cut” method, so that accurate measurements of absolute accessibility or occupancy at restriction sites throughout a genome are possible. The protocol is presented for the example of . chromatin but may be

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https://doi.org/10.1057/9781403980168, (Buenrostro et al, Nat Methods 10:1213–1218, 2013) modify lysis conditions to permeabilize cells and mitochondria, and (Corces et al, Nat Methods 14:959–962, 2017) optimize bioinformatic processing protocols to collectively increase mitochondrial genome coverage for downstream analysis. Here, we d

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1064-3745 expertsThis detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely

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Mapping Nucleosome Location Using FS-Seq with next-generation sequencing to map the location of nucleosomes in chromatin. In this section, a procedure using a kit from New England Biolabs (NEB NEXT Ultra II FS DNA library prep Kit) to fragment chromatin in preparation for next-generation sequencing is described and compared to other available procedures for mapping nucleosome location.