Titlebook: Chromatin Accessibility; Methods and Protocol Georgi K. Marinov,William J. Greenleaf Book 2023 The Editor(s) (if applicable) and The Author

slipped-disk

Medicaid

一美元

熱情贊揚(yáng)

American Doughboys and German ,,s to capture the dynamics governing methylation and accessibility vary across individual cells and cell types. Here, we provide a scalable plate-based protocol for preparing scNOMe-seq libraries from single nucleus suspensions.

antenna

American Doughboys and German ,,tational modifications existing single-cell ATAC-seq workflows but yields distinct modalities underlying complex cellular states, including estimation of protein abundance on the cell surface as well as intracellular and intranuclear factors.

constellation

Universal NicE-Seq: A Simple and Quick Method for Accessible Chromatin Detection in Fixed Cellsq (nicking enzyme-assisted sequencing). We also demonstrate a single tube method that enables direct NGS library preparation from low cell numbers without DNA purification. Furthermore, we demonstrated universal?NicE-seq on FFPE tissue section sample.

BATE

Simultaneous Measurement of DNA Methylation and Nucleosome Occupancy in Single Cells Using scNOMe-Ses to capture the dynamics governing methylation and accessibility vary across individual cells and cell types. Here, we provide a scalable plate-based protocol for preparing scNOMe-seq libraries from single nucleus suspensions.

indices

Massively Parallel Profiling of Accessible Chromatin and Proteins with ASAP-Seqtational modifications existing single-cell ATAC-seq workflows but yields distinct modalities underlying complex cellular states, including estimation of protein abundance on the cell surface as well as intracellular and intranuclear factors.