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Titlebook: Characterization of Plant Viruses; Methods and Protocol Alangar Ishwara Bhat,Govind Pratap Rao Book 2020 Springer Science+Business Media, L

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發(fā)表于 2025-3-21 17:24:29 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Characterization of Plant Viruses
副標(biāo)題Methods and Protocol
編輯Alangar Ishwara Bhat,Govind Pratap Rao
視頻videohttp://file.papertrans.cn/225/224009/224009.mp4
概述Includes comprehensive and complete practical manual containing protocols for identification, characterisation and detection of viruses infecting plants based on biological, physico-chemical, serologi
叢書名稱Springer Protocols Handbooks
圖書封面Titlebook: Characterization of Plant Viruses; Methods and Protocol Alangar Ishwara Bhat,Govind Pratap Rao Book 2020 Springer Science+Business Media, L
描述.This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on .in vitro. expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its
出版日期Book 2020
關(guān)鍵詞Plant Virology; Virus Characterization; Purification Protocols; Serological Methods; Biotechnological Ap
版次1
doihttps://doi.org/10.1007/978-1-0716-0334-5
isbn_softcover978-1-0716-0336-9
isbn_ebook978-1-0716-0334-5Series ISSN 1949-2448 Series E-ISSN 1949-2456
issn_series 1949-2448
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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,Spezielle Weierstra?sche Zeta-Funktionen,sect-proof conditions. This chapter provides information on some basic requirements and precautions essential for every plant virus worker to raise and maintain healthy nursery and virus cultures to carry out experiments under regulated environment and to prevent contamination.
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發(fā)表于 2025-3-22 06:34:51 | 只看該作者
,Spezielle Weierstra?sche Sigma-Funktionen,temic infections in susceptible plants. Symptoms caused by viruses fall into two categories, namely internal and external. In the present chapter, both external and internal symptoms caused by viruses are explained.
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發(fā)表于 2025-3-22 10:05:39 | 只看該作者
https://doi.org/10.1007/978-3-662-36381-2nts. Although, these properties are not being used at present to identify and characterize a plant virus, but still they are quite useful in getting idea about concentration and storage duration of a virus in active state in crude sap for future course of study.
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Statistische Signalverarbeitung, different modes: non-persistent (stylet-borne), semi-persistent, persistent and persistent propagative. Majority of aphid species are involved in the transmission of non-persistent or stylet-borne viruses.
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Physico-chemical Properties of Virus in Crude Sap,nts. Although, these properties are not being used at present to identify and characterize a plant virus, but still they are quite useful in getting idea about concentration and storage duration of a virus in active state in crude sap for future course of study.
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