| 書目名稱 | Characterization of Plant Viruses | | 副標題 | Methods and Protocol | | 編輯 | Alangar Ishwara Bhat,Govind Pratap Rao | | 視頻video | http://file.papertrans.cn/225/224009/224009.mp4 | | 概述 | Includes comprehensive and complete practical manual containing protocols for identification, characterisation and detection of viruses infecting plants based on biological, physico-chemical, serologi | | 叢書名稱 | Springer Protocols Handbooks | | 圖書封面 |  | | 描述 | .This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on .in vitro. expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its | | 出版日期 | Book 2020 | | 關鍵詞 | Plant Virology; Virus Characterization; Purification Protocols; Serological Methods; Biotechnological Ap | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-0334-5 | | isbn_softcover | 978-1-0716-0336-9 | | isbn_ebook | 978-1-0716-0334-5Series ISSN 1949-2448 Series E-ISSN 1949-2456 | | issn_series | 1949-2448 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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