Titlebook: Cellular and Molecular Aspects of the Plant Hormone Ethylene; Proceedings of the I Jean Claude Pech,Alain Latché,Claudine Balagué Conferenc

CODE

Cloning and Expression Analysis of an , 1-Aminocyclopropane-1-Carboxylate Synthase Gene: Pattern of by the root transformation method. Temporal and spatial regulation of expression of . were analyzed, revealing a developmental control both in shoot and root and confirming results obtained by RT-PCR analysis. A role for . in the developmental control of ethylene synthesis is suggested.

濃縮

Immunocytolocalisation of ACC Oxidase in Tomato Fruitsbodies were purifed by affinity column chromatography and eventually saturated with excess of antigenes. Pericarp tissue of both normal and transgenic tomatoes transformed with antisense pTOM13 cDNA (kindly provided by A Hamilton and D. Grierson, Nottingham, UK) were processed for immunocytolocalization as described in [4].

esculent

Monomeric and Dimeric Forms and Mechanism-Based Inactivation of 1-Aminocyclopropane-1-Carboxylate Synthases have a property to form a dimer, but in tomato fruit tissue some modification takes place to the enzyme protein, which makes it remained as a monomer and less sensitive to the mechanism-based inactivation.

mydriatic

Maximising the Activity of the Ethylene-Forming Enzyme the EFE activity from melon. The EFE activity is also stimulated by the addition of HCO../CO. to the reaction mixture, with a 25-fold increase in activity for melon and an 8-fold increase in activity for apple.

砍伐

無(wú)聊點(diǎn)好

LIMN

https://doi.org/10.1007/978-3-540-79332-8ery of EFE activity from the cell extracts. Using residue-specific chemical modification, a histidine critical for catalytic activity was detected. This is likely to be one of three histidine residues highly conserved among sequence-related Fe(II) ascorbate-dependent oxidases.

暗諷

Purification and Characterization of ACC Oxidase and Its Expression during Ripening in Apple Fruiteclimacteric apples. Immunoblots of proteins and of . translation products of poly(A)+ RNA from apples at progressive stages of the ethylene climacteric indicated that ACC oxidase and the mRNA encoding ACC oxidase was nil in unripe fruits but accumulated markedly during the ethylene climacteric.

偶像

Mechanistic Aspects of ACC Oxidation to Ethylenee (HPA), might occur. HPA has been found not only in stepwise-type reactions, such as those mediated by peroxidase, but also in concerted reactions, such as the oxidation by hypochlorite or hydrogen peroxide. In this work we investigate the mechanism of ACC oxidation by the two kinds of reactions and discuss some physiological implications.

CHYME