Titlebook: Caveolae; Methods and Protocol Cedric M. Blouin Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 electron micro

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Analysis of Protein and Lipid Interactions Using Liposome Co-sedimentation Assays,lae at the cell surface via coat proteins. The liposome co-sedimentation assay has been widely used for studies of protein and lipid interactions and has provided important information about binding mechanisms, lipid-binding specificity, and curvature preference of proteins. Here, we describe this t

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Preparation of Caveolin-1 for NMR Spectroscopy Experiments,-stresses on the cell, endocytosis, and most importantly caveolae formation. As a consequence, there is intense interest in characterizing caveolin-1 structurally. Out of the many available structural techniques, nuclear magnetic resonance (NMR) spectroscopy is particularly well suited to investigat

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大酒杯

Pulling of Tethers from the Cell Plasma Membrane Using Optical Tweezers,easuring the force required to hold the membrane tether at a constant length, which is related to the cell membrane tension. Following the evolution of this force during mechanical or chemical perturbations of the cell gives insight about the regulation of cell membrane tension. By pulling very long

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Live Confocal Imaging of Zebrafish Notochord Cells Under Mechanical Stress In Vivo,sing the zebrafish notochord as a manipulable experimental system. Here, the methodologies to prepare, label, and simultaneously induce and image mechanical loading on live zebrafish notochord cells via electrical stimulation are described. This approach investigates membrane mechanics in a live, ph

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Study of Caveolae-Dependent Mechanoprotection in Human Muscle Cells Using Micropatterning and Live-en developed to study caveolae-dependent mechanoprotection and had to be adapted to the tissue or cells studied, as these structures are found in almost every type of cells. This chapter focuses on a protocol combining the use of live-cell imaging, micropatterning, hypo-osmotic shock as a mechanical

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