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Titlebook: Caveolae; Methods and Protocol Cedric M. Blouin Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 electron micro

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書目名稱Caveolae
副標題Methods and Protocol
編輯Cedric M. Blouin
視頻videohttp://file.papertrans.cn/223/222675/222675.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Caveolae; Methods and Protocol Cedric M. Blouin Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 electron micro
描述.This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Caveolae: Methods and Protocols. is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and protein
出版日期Book 2020
關(guān)鍵詞electron microscopy; protein purification; fluorescence microscopy; spinning disk microscopy; endothelia
版次1
doihttps://doi.org/10.1007/978-1-0716-0732-9
isbn_softcover978-1-0716-0734-3
isbn_ebook978-1-0716-0732-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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Book 2020e laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Caveolae: Methods and Protocols. is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and protein
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Bezugsrahmen und Hypothesenbildung,t of the plasma membrane. From these partitions, we estimate the relative fraction of caveolin that is punctate versus diffuse. Finally, we analyze the mobility of caveolin aggregates by tracking them and classify individual trajectories as diffusive or subdiffusive using a moment scaling spectrum a
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978-1-0716-0734-3Springer Science+Business Media, LLC, part of Springer Nature 2020
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