Titlebook: Cartilage Tissue Engineering; Martin J. Stoddart,Elena Della Bella,Angela R. Arm Book 2023 The Editor(s) (if applicable) and The Author(s)

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Chondrocyte Isolation and Expansion,acted primary chondrocytes will then adhere to standard tissue culture plastics, typically in small clusters, over a period of a few days in monolayer culture. Chondrocyte populations are expanded in a basal medium containing serum, supplemented with ascorbic acid, antibiotics, and sometimes antifun

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Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation, maintenance, and repair. In this chapter, we show how colony-forming progenitor-like cells can be isolated from bovine articular cartilage using differential adhesion to fibronectin. Furthermore, we describe the optimal conditions and factors required to differentiate these progenitor cells to prod

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Physioxic Culture of Chondrogenic Cells, between 2% and 5% oxygen. Although the literature has historically termed this level of oxygen as hypoxia, particularly when doing experiments in vitro in this range, this is actually the physiological oxygen tension experienced in vivo and is more accurately termed physioxia. In general, culture o

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Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering. This makes them ideal candidates for treating focal cartilage defects and cartilage degeneration in osteoarthritis (OA). Observational studies have reported beneficial biological effects of EVs, such as inhibition of inflammation, enhanced extracellular matrix deposition, and reduced cartilage d

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