Titlebook: Cancer Cell Culture; Methods and Protocol Dania Movia,Adriele Prina-Mello Book 2023 The Editor(s) (if applicable) and The Author(s), under

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Generation of Radioresistant Prostate Cancer Cellsate the mechanisms of radioresistance in cancer cells. Owing to the complex nature of the biological effect of ionizing radiation, the generation and validation of these models requires the careful consideration of radiation exposure protocols and cellular endpoints. This chapter presents a protocol

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Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocarlay treatment resistance. The molecular pathways leading to treatment resistance in cancer have been subject to research for many years. Isogenic cell lines with divergent radiosensitivities are an extremely useful tool to study the molecular mechanisms that underpin radioresistance in cancer resear

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Ossification

A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air–Liquid Interfacein the investigation of tumor growth and proliferation, invasion, and drug screening. However, 3D tumor spheroids and tumoroids cannot fully mimic the architecture of the human lung adenocarcinoma tissue and, in particular, the direct contact of the lung adenocarcinoma cells with the air, as they la

Climate

Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cource of patient-specific cancer cells, as well as non-malignant cells, for research proposes. The simple and reproducible method presented here allows isolation of viable mononuclear cells, including malignant cells, from fresh peripheral blood or bone marrow aspirates using density gradient centri

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bromide

Diluge

A Method for the In Vitro Cytotoxicity Assessment of Anti-cancer Compounds and Materials Using High tial toxicity. This issue not only causes a high attrition rate for these compounds but also slows down the drug discovery process in general. To overcome this problem, robust, accurate, and reproducible methodologies for assessing anti-cancer compounds are essential. Multiparametric technique and h