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Titlebook: Cancer Cell Culture; Methods and Protocol Dania Movia,Adriele Prina-Mello Book 2023 The Editor(s) (if applicable) and The Author(s), under

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發(fā)表于 2025-3-21 19:51:32 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Cancer Cell Culture
副標(biāo)題Methods and Protocol
編輯Dania Movia,Adriele Prina-Mello
視頻videohttp://file.papertrans.cn/222/221059/221059.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Cancer Cell Culture; Methods and Protocol Dania Movia,Adriele Prina-Mello Book 2023 The Editor(s) (if applicable) and The Author(s), under
描述.This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two contains technical chapters that illustrate step-by-step methodologies for specific cancer cell culture methods. The methods discussed range from the generation of isogenic cancer cell lines, the use of serum-free growth conditions, and three-dimensional cell cultures and their specific assays for the efficacy assessment of new anticancer therapies. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Cancer Cell Culture: Methods and Protocols. is a valuable tool to
出版日期Book 2023
關(guān)鍵詞3D tumour models; Tumoroids; Stem Cells; 2D Cell Cultures; Angiogenesis
版次1
doihttps://doi.org/10.1007/978-1-0716-3056-3
isbn_softcover978-1-0716-3058-7
isbn_ebook978-1-0716-3056-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

書目名稱Cancer Cell Culture影響因子(影響力)




書目名稱Cancer Cell Culture影響因子(影響力)學(xué)科排名




書目名稱Cancer Cell Culture網(wǎng)絡(luò)公開度




書目名稱Cancer Cell Culture網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Cancer Cell Culture被引頻次




書目名稱Cancer Cell Culture被引頻次學(xué)科排名




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書目名稱Cancer Cell Culture年度引用學(xué)科排名




書目名稱Cancer Cell Culture讀者反饋




書目名稱Cancer Cell Culture讀者反饋學(xué)科排名




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發(fā)表于 2025-3-21 22:35:40 | 只看該作者
Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Esophageal Adenocar by chronic irradiation of esophageal adenocarcinoma cells with clinically relevant doses of X-ray radiation. We also characterize cell cycle, apoptosis, reactive oxygen species (ROS) production, DNA damage and repair in this model to investigate the?underlying molecular mechanisms of radioresistance in esophageal adenocarcinoma.
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發(fā)表于 2025-3-22 05:08:50 | 只看該作者
Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancerenge for oncologists. In order to study and elucidate the cellular and molecular mechanisms implicated in the development of drug resistance in cancer, the use of isogenic models provides a valuable in vitro tool for investigating novel biomarkers and the identification of potential targetable pathways involved in drug-resistant cancers.
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發(fā)表于 2025-3-22 12:25:28 | 只看該作者
Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environmentading to possible reproducibility issues in experiments and readouts. This chapter describes how to transition A549 cells to FBS-free medium and gives some insights on the further characterizations and functionality assays that would be necessary to perform for the validation of the cultured cells.
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發(fā)表于 2025-3-22 13:05:19 | 只看該作者
A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air–Liquid Interfaceck polarity. Our method allows to overcome this limitation by enabling to grow tumoroids of lung adenocarcinoma cells and healthy lung fibroblasts at the Air–Liquid Interface (ALI). This ensures straightforward access to both the apical and basal surface of the cancer cell culture, with several advantages in drug screening applications.
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發(fā)表于 2025-3-22 20:57:50 | 只看該作者
Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cfugation. The cells obtained using the protocol described can be further purified for a variety of cellular, immunological, molecular, and functional assays. In addition, these cells can be cryopreserved and bio-banked for future research studies.
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發(fā)表于 2025-3-23 08:38:38 | 只看該作者
Joan P. Schwartz,Ann M. Marini,R. W. Rodieck, we describe a methodology to generate 3D liver spheres from pluripotent stem cells. Liver spheres are?composed?of?three key liver cell types (hepatic progenitor cells, endothelial cells, and hepatic stellate cells)?and?have been used to study human cancer?cell metastasis.
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