Titlebook: CRISPR; Methods and Protocol Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fi Book 2015 Springer Science+Business Media New York 2015 CRI

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書目名稱CRISPR影響因子(影響力)




書目名稱CRISPR影響因子(影響力)學科排名




書目名稱CRISPR網(wǎng)絡公開度




書目名稱CRISPR網(wǎng)絡公開度學科排名




書目名稱CRISPR被引頻次




書目名稱CRISPR被引頻次學科排名




書目名稱CRISPR年度引用




書目名稱CRISPR年度引用學科排名




書目名稱CRISPR讀者反饋




書目名稱CRISPR讀者反饋學科排名

misanthrope

Musket

GRAZE

休戰(zhàn)

Samiran Bera,Santosh Kumar Das,Joydev Ghoshs a metal-dependent endonuclease activity. Here we describe the protocols for the analysis of nuclease activity of purified Cas1 proteins against various DNA substrates including Holliday junctions and other intermediates of DNA recombination and repair.

吊胃口

吊胃口

In Vitro Co-reconstitution of Cas Protein Complexes,roduced as inactive inclusion bodies. Here, we present a detailed protocol for the isolation and purification of insoluble Cas proteins. Guidelines for their solubilization via co-reconstitution strategies and procedures to upscale the production of soluble multimeric Cas protein complexes are provided.

種屬關(guān)系

Rapid Multiplex Creation of , Strains Capable of Interfering with Phage Infection Through CRISPR,,” can be used for very simple and rapid construction of multiple . strains capable of targeting, through CRISPR interference, any phage or plasmid of interest. Availability of such strains should allow rapid progress in the analysis of CRISPR-Cas system function against diverse mobile genetic elements.

暗語

Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes,es of crRNA-Cas complexes can be quantified by calculating the dissociation constant (..). Here, we describe how two types of EMSA assays are performed using the Cascade ribonucleoprotein complex from . as an example.

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