Titlebook: Biological Electron Microscopy; Theory, Techniques, Michael J. Dykstra,Laura E. Reuss Textbook 2003Latest edition Michael J. Dykstra 2003

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Techniques, fixation, very few solvents, and no resins, and avoids the heating process normally utilized to polymerize many embedding media. The procedure is quick and is one of the best ways to produce materials for consequent intracellular immunolabeling.

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Cytochemistry,chapter is to introduce some of the different general types of staining procedures frequently employed to demonstrate specific chemical entities associated with cellular surfaces and cytoplasmic contents.

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Vacuum Systems,nt types of vacuum sensors, or gauges, and vacuum pumps utilized in electron microscopy. This is especially important because various gauges and pumps can sustain damage if connected to the system being evacuated at an improper vacuum level.

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Anne Kiefhaber,Willis B. Goldbeckor stick, as follows: heat dissecting needle tip to red hot with a Bunsen burner and . burn a small Channel in the tip of the applicator stick (Fig. 101C). Next, insert the end of the nichrome wire into the burned Channel and secure it by applying a small drop of 5-min epoxy to the assembly (Fig. 101D).