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Titlebook: Biological Electron Microscopy; Theory, Techniques, Michael J. Dykstra,Laura E. Reuss Textbook 2003Latest edition Michael J. Dykstra 2003

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期刊全稱Biological Electron Microscopy
期刊簡(jiǎn)稱Theory, Techniques,
影響因子2023Michael J. Dykstra,Laura E. Reuss
視頻videohttp://file.papertrans.cn/188/187355/187355.mp4
發(fā)行地址Covers a wide range of topics including conventional light microscopy, transmission electron microscopy, and scanning electron microscopy.Includes a survey of each subject, and a techniques section, w
圖書封面Titlebook: Biological Electron Microscopy; Theory, Techniques,  Michael J. Dykstra,Laura E. Reuss Textbook 2003Latest edition Michael J. Dykstra 2003
影響因子Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti- cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated
Pindex Textbook 2003Latest edition
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書目名稱Biological Electron Microscopy影響因子(影響力)




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Techniques, The technique was pioneered by ., . and has since become a Standard technique (.). The strength o f the Tokuyasu technique is that it employs minimal fixation, very few solvents, and no resins, and avoids the heating process normally utilized to polymerize many embedding media. The procedure is qui
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Cytochemistry,h reagent and label penetration of tissues and cells, and problems distinguishing reaction products from other cellular constituents. The aim of this chapter is to introduce some of the different general types of staining procedures frequently employed to demonstrate specific chemical entities assoc
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Techniques,tages of colloidal gold techniques is that the gold particles cannot be confused with any other cellular constituents, unlike the products of previously discussed ferritin and peroxidase/DAB/osmium staining procedures.
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