Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2007Latest edition Humana Press 2007 Expression.Protein.cell lin

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Handbuch Innovative Wirtschaftsf?rderungf the viral specific expression cassette with a mammalian expression cassette or the addition of a mammalian expression cassette. Viruses are produced using standard methods in a few weeks. Mammalian cells transduced with the BacMam viruses have been routinely used as substitutes for stable cell lines.

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Handbuch Innovative Wirtschaftsf?rderung and long-term (frozen in liquid nitrogen) storage of cell lines, as well as quality control procedures for the cultures. Methods for storing baculoviruses for use in cell cultures and issues of concern when using cell cultures for their production and study are also described.

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1064-3745 l lines and provides methods for isolating new cell lines.Nu.Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides a

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Introduction to Baculovirus Molecular Biologyation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure, and their replication in insect larvae. Baculovirus gene expression occurs in an ordered cascade, regulated by early, la

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Baculovirus Transfer Vectorshen cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available. It is impractical to list every transfer vector that has ever been used. Instead, we focus on those that are available commer

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Recombinant Baculovirus Isolationge in which insect cells are transfected with either the virus genome alone (Bac-to-Bac? or BaculoDirect?, Invitrogen) or virus genome and transfer vector. In the latter case, this allows the natural process of homologous recombination to transfer the foreign gene, under control of the polyhedrin or