Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2007Latest edition Humana Press 2007 Expression.Protein.cell lin

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Development of Serum-Free Media for Lepidopteran Insect Cell Linesion systems and is increasingly used industrially on a large scale. Therefore, there is a constant need for convenient and low-cost culture media capable of supporting good insect cell growth and ensuring high yield of baculovirus as well as the strong expression of recombinant proteins. Vertebrate

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Small-Scale Protein Production With the Baculovirus Expression Vector Systemtem (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and express

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Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression . Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, metho

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Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insecnsect cell system. The production and scale-up of these particles, which are mostly sought as candidate vaccines, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. Recently, adeno-associated viral (AAV) vectors, which can be pot

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Baculovirus and Insect Cell Expression Protocols978-1-59745-457-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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