Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial

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Generation of Orthogonally Selective Bacterial Riboswitches by Targeted Mutagenesis and In Vivo Scrnd library to identify functional riboswitch–ligand combinations. Promising riboswitch–ligand pairs are then further characterized both in vivo and in vitro. Using this method, a series of artificial riboswitches can be generated that are versatile synthetic biology tools for use in protein producti

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Nucleotide Kinase-Based Selection System for Genetic Switches,ompleted only by liquid handling, facilitating the parallel and continuous operations of multiple selection projects. This automation-liable platform for genetic selection of functional switches has potential applications for development of RNA-based biosensors, expression controllers, and their int

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,Measuring Riboswitch Activity In Vitro and in Artificial Cells with Purified Transcription–Translatd system can be easily reconstituted within compartments to build artificial, cellular mimics with sensing capability, protocols are provided for building sense-response systems within water-in-oil emulsion compartments and lipid vesicles. Only standard laboratory equipment and commercially availabl

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1064-3745 reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Artificial Riboswitches: Methods and Protocols. seeks to serve not only bioengineers who 978-1-4939-6330-0978-1-62703-755-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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