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Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial

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發(fā)表于 2025-3-21 16:46:38 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
期刊全稱Artificial Riboswitches
期刊簡稱Methods and Protocol
影響因子2023Atsushi Ogawa
視頻videohttp://file.papertrans.cn/163/162721/162721.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial
影響因子.Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules.. Artificial Riboswitches: Methods and Protocols. focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Artificial Riboswitches: Methods and Protocols. seeks to serve not only bioengineers who
Pindex Book 2014
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發(fā)表于 2025-3-21 21:47:34 | 只看該作者
Fictional Worlds and the Moral Imaginationer with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique to discover artificial riboswitches in .. . cells that is based on TEV protease–FRET substrate reporter system.
板凳
發(fā)表于 2025-3-22 02:03:41 | 只看該作者
Fictional Worlds and the Moral Imaginationescribe a library screening strategy for efficient creation of ON riboswitches of . of .. An ON riboswitch of . is then integrated with the . promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.
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FRET-Based Optical Assay for Selection of Artificial Riboswitches,er with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique to discover artificial riboswitches in .. . cells that is based on TEV protease–FRET substrate reporter system.
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發(fā)表于 2025-3-23 01:05:39 | 只看該作者
Portable Two-Way Riboswitches: Design and Engineering,escribe a library screening strategy for efficient creation of ON riboswitches of . of .. An ON riboswitch of . is then integrated with the . promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.
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發(fā)表于 2025-3-23 04:26:28 | 只看該作者
Engineering Protein-Responsive mRNA Switch in Mammalian Cells,ruct a genetic circuit. Insertion of a protein binding RNA sequence in the 5′ UTR of mRNA would allow for the generation of a protein-responsive RNA switch. Here we describe the design principle of the switch and methods for tuning and analyzing its translational activity in mammalian cells.
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發(fā)表于 2025-3-23 08:29:50 | 只看該作者
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