Titlebook: Archaea; Methods and Protocol Sébastien Ferreira-Cerca Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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ChIP-Seq Occupancy Mapping of the Archaeal Transcription Machineryof many established archaeal model organisms necessitates further adaptations. This chapter describes a version of ChIP-seq adapted for the basal transcription machinery of thermophilic archaea and some modifications to the data analysis.

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https://doi.org/10.1007/978-1-4615-1593-7es found in the CRISPR arrays, effectively suppress unspecific DNA and RNA activities of the CRISPR-Cas complexes. Here we describe the use of endogenous type III CRISPR-Cas systems in two model organisms of Crenarchaeota, . and ..

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Alexis Tsoukiàs,Herimandimbiniaina Ralijaonaof many established archaeal model organisms necessitates further adaptations. This chapter describes a version of ChIP-seq adapted for the basal transcription machinery of thermophilic archaea and some modifications to the data analysis.

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1064-3745 eshooting and known pitfalls, and step-by-step, readily reproducible protocols..Authoritative and cutting-edge, .Archaea: Methods and Protocols. aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field..978-1-0716-2447-0978-1-0716-2445-6Series ISSN 1064-3745 Series E-ISSN 1940-6029