Titlebook: Archaea; Methods and Protocol Sébastien Ferreira-Cerca Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaearoduction of the . gene on a plasmid leads to a selectable loss of 8-ADP resistance after a homologous recombination event (pop-in). A subsequent pop-out event restores the 8-ADP resistance and can generate chromosomal mutants with frequencies of about 50%.

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Proteome Turnover Analysis in , by a Heavy Isotope Multilabeling Approachthat allowed the successful analysis of relative protein synthesis and degradation rates on the proteome scale of the halophilic archaeon .. This method combines .N and .C isotope metabolic labeling with high-resolution mass spectrometry and data analysis tools (QuPE web-based platform) and could be applied to different archaea.

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Assessment for conservation and protection,article gives a step-by-step introduction on how to use the implemented system for any gene of interest in . .. The concept of CRISPRi described here for . can be transferred to any other archaeon, that is genetically tractable and has an endogenous CRISPR-Cas I systems.

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F. Schmitten,K.-H. Schepers,A. Festerlingonal DNA transfer from . to . ΔH with shuttle-vector plasmid DNA, which allows the genetic manipulation of this microbe, and provides a basis for the development of further genetic methods for this and potentially other representatives of Methanobacteriales.

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