| 期刊全稱 | Antiviral Resistance in Plants | | 期刊簡稱 | Methods and Protocol | | 影響因子2023 | John M. Watson,Ming-Bo Wang | | 視頻video | http://file.papertrans.cn/159/158709/158709.mp4 | | 發(fā)行地址 | Details many of the methods currently used to study RNA silencing in relation to viral infections in plants.Features step-by-step, easy to follow protocols from an international assortment of laborato | | 學(xué)科分類 | Methods in Molecular Biology | | 圖書封面 |  | | 影響因子 | .Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In .Antiviral Resistance in Plants: Methods and Protocols., expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.? These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.? Written in the highly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troublesho | | Pindex | Book 2012 |
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